Effect of repeatedin vitrosub-culturing on the virulence ofMetarhizium anisopliaeagainstHelicoverpa armigera(Lepidoptera: Noctuidae)
| Autor: | Pallavi B. Nahar, Armugham Rajendran, Yogesh S. Shouche, Mukund V. Deshpande, Santosh B. Chavan, Mahesh Kulye, Girish Kulkarni, Priya Yadav, S. Kulkarni |
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| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Biocontrol Science and Technology. 18:337-355 |
| ISSN: | 1360-0478 0958-3157 |
| DOI: | 10.1080/09583150801935650 |
| Popis: | The effect of repeated conidial sub-culturing of Metarhizium anisopliae on its virulence against Helicoverpa armigera (Hubner) was studied. The LT50 observed against third instar larvae of H. armigera for the first sub-culture was 3.4 days; it increased to 4.5 and 5.6 days for the 20th and the 40th sub-cultures, respectively. The LT50 values after passage of the 40th sub-culture on H. armigera decreased to 4.4 and 3.7 days for the 40th (first in vivo) and the 40th (fifth in vivo) passages, respectively. Similarly, the LC50 of M. anisopliae towards third instar larvae of H. armigera increased from the first sub-culture (0.17×104) to (3.0×104) for the 40th conidial transfers on potato dextrose agar and again decreased to 0.74×104 and 0.23×104 in the 40th (first in vivo) and the 40th (fifth in vivo) passage, respectively. Similar trends for LC50 and LT50 values were seen when sugarcane woolly aphid, Ceratovacuna lanigera Zehntner was used as a host. Significant variation in appressorium formation an... |
| Databáze: | OpenAIRE |
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