Collection of Mitochondrial tRNA Sequences and Anticodon Identification for Acheta domesticus
| Autor: | Yash Munnalal Gupta, Kittisak Buddhachat, Surin Peyachoknagul, Somjit Homchan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Mitochondrial DNA Materials science biology Mechanical Engineering Condensed Matter Physics biology.organism_classification 010603 evolutionary biology 01 natural sciences Mitochondrial trna 03 medical and health sciences Mechanics of Materials Phylogenetics Acheta Transfer RNA General Materials Science Identification (biology) 030304 developmental biology |
| Zdroj: | Materials Science Forum. 967:65-70 |
| ISSN: | 1662-9752 |
| Popis: | The mitochondria are organelles found within eukaryotic cell, possess own small circular DNA (mtDNA) apart from the most of DNA found in cell nucleus. The transcription and translation of mtDNA requires tRNA that often encoded by mtDNA itself. The mtDNA evolves faster than genomic DNA primary due to mitochondrial dysfunction and pathogenesis. The genes of mitochondria tRNA (mt tRNA) are prone to mutate that links to mitochondrial activity and protein synthesis machinery. It is important to understand the codon use by mt tRNA for Acheta domesticus to understand evolutionary relationship within closely related species and mitochondrial protein synthesis machinery. The present study uses the High throughput RNA sequencing data to identify mt tRNA genes using to examine the codon use for mitochondrial protein synthesis process. The conservative property of tRNA secondary structure assisted identified and confirmed anchored tRNA sequences with respective amino acid anticodon according to genetic code for tRNA in mtDNA. This study provides mt tRNA sequences to understand evolution of mitochondrial tRNA of Acheta domesticus with other related species to establish phylogeny. Moreover, mt tRNAs are the exons that provides partial sequences for mitochondria DNA. The novel approach for tRNA identification will guide other studies for PCR free in silico analysis. |
| Databáze: | OpenAIRE |
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