Sensitivity of mitochondrial DNA depleted ρ0 cells to H2O2 depends on the plasma membrane status
| Autor: | Yoshikazu Kuwahara, Tomoaki Sato, Yoshihiro Nishitani, Yuko Takashi, Manabu Fukumoto, Takao Tsukahara, Akihiro Kurimasa, Kazuo Tomita |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
inorganic chemicals
0301 basic medicine Membrane potential Membrane permeability Biophysics Cell Biology Biology biology.organism_classification Biochemistry Molecular biology Cell biology HeLa 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Cell culture Catalase 030220 oncology & carcinogenesis Extracellular biology.protein Hydrogen peroxide Molecular Biology Intracellular |
| Zdroj: | Biochemical and Biophysical Research Communications. 490:330-335 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.06.044 |
| Popis: | To clarify the relationship between mitochondrial DNA (mtDNA)-depleted ρ0 cells and the cellular sensitivity to hydrogen peroxide (H2O2), we established HeLa and SAS ρ0 cell lines and investigated their survival rate in H2O2, radical scavenging enzymes, plasma membrane potential status, and chronological change in intracellular H2O2 amount under the existence of extracellular hydrogen peroxide compared with the parental cells. The results revealed that ρ0 cells had higher sensitivity to H2O2 than their parental cells, even though the catalase activity of ρ0 cells was up-regulated, and the membrane potential of the ρ0 cells was lower than their parental cells. Furthermore, the internal H2O2 amount significantly increased only in ρ0 cells after 50 μM H2O2 treatment for 1 h. These results suggest that plasma membrane status of ρ0 cells may cause degradation, and the change could lead to enhanced membrane permeability to H2O2. As a consequence, ρ0 cells have a higher H2O2 sensitivity than the parental cells. |
| Databáze: | OpenAIRE |
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