Rapid and sensitive determination of major polyphenolic components inEuphoria longanaLam. seeds using matrix solid-phase dispersion extraction and UHPLC with hybrid linear ion trap triple quadrupole mass spectrometry

Autor:	Kakasaheb R. Mahadik, Atul S. Rathore, L. Sathiyanarayanan, Shreekant Deshpande
Rok vydání:	2016
Předmět:	Chromatography Formic acid Electrospray ionization 010401 analytical chemistry Extraction (chemistry) Analytical chemistry Filtration and Separation 04 agricultural and veterinary sciences Mass spectrometry 040401 food science 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Triple quadrupole mass spectrometer Matrix (chemical analysis) chemistry.chemical_compound 0404 agricultural biotechnology chemistry Quadrupole ion trap
Zdroj:	Journal of Separation Science. 39:4335-4343
ISSN:	1615-9306
DOI:	10.1002/jssc.201600685
Popis:	A rapid and sensitive method for the extraction and determination of four major polyphenolic components in Euphoria longana Lam. seeds is presented for the first time based on matrix solid-phase dispersion extraction followed by ultra high performance liquid chromatography with hybrid triple quadrupole linear ion trap mass spectrometry. Matrix solid-phase dispersion method was designed for the extraction of Euphoria longana seed constituents and compared with microwave-assisted extraction and ultrasonic-assisted extraction methods. Ultra performance liquid chromatography-hybrid triple quadrupole linear ion-trap mass spectrometry method was developed for quantitative analysis in multiple-reaction monitoring mode in negative electrospray ionization. The chromatographic separation was accomplished using an ACQUITY UPLC BEH C18 (2.1 mm × 50 mm, 1.7 μm) column with gradient elution of 0.1% aqueous formic acid and 0.1% formic acid in acetonitrile. The developed method was validated with acceptable linearity (r2 > 0.999), precision (RSD ≤ 2.22%) and recovery (RSD ≤ 2.35%). The results indicated that matrix solid-phase dispersion produced comparable extraction efficiency compared with other methods nevertheless was more convenient and time-saving with reduced requirements on sample and solvent volumes. The proposed method is rapid and sensitive in providing a promising alternative for extraction and comprehensive determination of active components for quality control of Euphoria longana products. This article is protected by copyright. All rights reserved
Databáze:	OpenAIRE
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