| Autor: |
Yao Tong, Wen-jun Wan, Han Yang, Beng-gang You, Yu Wang, Xue-nong Zhang, Dan-dan Wang, Yang Liu |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-20688/v1 |
| Popis: |
Backgroud: Gene therapy has attracted widespread attention as a potential method of treating some autoimmune diseases, genetic diseases and cancer. It is critical to discover a new nanocarrier for the effectiveness of gene therapy. Reportedly, the dual-targed nano-delivery system as a novel strategy could improve targeting efficiency and deliver more drugs or genes to tumor sites over current single-targed nano-delivery delivery system.Methods: We synthesized and characterized the hyaluronic acid (HA)-folic acid (FA) polymer, and then prepared the cationic liposomes by ultrasonic dispersion . The liposome was coated with HA-FA to obtain HA-FA-Lip nanoparticles. HA-FA-Lip formed complexes with siRNA Bcl-2 (siBcl-2) through electrostatic adsorption for delivering siBcl-2 to the tumor site.Results: HA-FA-Lip showed stronger tumor accumulation and better targeting efficiency than HA-lip. Meanwhile, HA-FA-Lip delivered more siBcl-2 to tumor cells and played a role in down-regulating Bcl protein levels, thus inducing obvious apoptosis.Conclusions: As a new type of dual-targeted drug delivery system, HA-FA-Lip was expected to be an effective nanocarrier with high transfection efficiency and good biocompatibility. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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