Two-site immunoassay of recombinant hirudin based on two monoclonal antibodies

Autor:	B. Mille, Claudine Picard, O. Condamines, D. Dussossoy, Jean-Marc Herbert, Mohamed Maftouh, Bernard Pau
Rok vydání:	1994
Předmět:	Detection limit medicine.diagnostic_test Chemistry medicine.drug_class Biochemistry (medical) Clinical Biochemistry Hirudin Monoclonal antibody Biological fluid law.invention Recombinant Hirudin Biochemistry law Immunoassay medicine Recombinant DNA Quantitative analysis (chemistry) medicine.drug
Zdroj:	Clinical Chemistry. 40:734-739
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/40.5.734
Popis:	Two monoclonal antibodies (mAbs), 10-2 and 10-5, both directed against recombinant hirudin variant 2-Lys47 (rHV2), were selected for their high affinity and epitopic specificities to develop a two-site immunoassay of rHV2. The mAb concentrations, incubation time, and temperature were optimized. The immunoassay has a detection limit for rHV2 of 45 ng/L in plasma and 30 ng/L in urine. The reactivity of the mAbs was tested against rHV2 and several forms of this protein truncated in the carboxyl terminus. The capture mAb 10-2 was found to be mainly directed against rHV2, whereas tracer mAb 10-5 was independent of the carboxyl-terminal region of the protein. This explains the high specificity of the immunoassay for the 65-amino acid form of hirudin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3144c917fd1168155fe55189134beb8e https://doi.org/10.1093/clinchem/40.5.734 Zobrazit plný text záznamu