Comparison of cobasEGFRMutation Test v2 and PANAMutyper-R-EGFRfor Detection and Semi-Quantification of Epidermal Growth Factor Receptor Mutations in Plasma and Pleural Effusion Supernatant
| Autor: | A-Lum Han, Moxin Wu, Keum-Ha Choi, Young Jin Lee, Yuya Huang, Min-Cheol Park, Do-Sim Park, Ki-Eun Hwang, Mengyu Zhu, Ji-Hyun Cho, Hak-Ryul Kim |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Chemistry Pleural effusion Concordance Biochemistry (medical) Clinical Biochemistry General Medicine medicine.disease Molecular biology Confidence interval 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Blood plasma Genotype medicine biology.protein Epidermal growth factor receptor Lung cancer Quantitative analysis (chemistry) |
| Zdroj: | Annals of Laboratory Medicine. 39:478-487 |
| ISSN: | 2234-3814 2234-3806 |
| DOI: | 10.3343/alm.2019.39.5.478 |
| Popis: | BACKGROUND Plasma epidermal growth factor receptor (EGFR) mutation tests are less invasive than tissue EGFR mutation tests. We determined which of two kits is more efficient: cobas EGFR Mutation test v2 (cobasv2; Roche Molecular Systems, Pleasanton, CA, USA) or PANAMutyper-R-EGFR (Mutyper; Panagene, Daejeon, Korea). We also evaluated whether pleural effusion supernatant (PE-SUP) samples are assayable, similar to plasma samples, using these two kits. METHODS We analyzed 156 plasma and PE-SUP samples (31 paired samples) from 116 individuals. We compared the kits in terms of accuracy, assessed genotype concordance (weighted κ with 95% confidence intervals), and calculated Spearman's rho between semi-quantitatively measured EGFR-mutant levels (SQIs) measured by each kit. We also compared sensitivity using 47 EGFR-mutant harboring samples divided into more-dilute and less-dilute samples (dilution ratio: ≥ or |
| Databáze: | OpenAIRE |
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