Encapsulation of 5-Flurouracil into PLGA Nanofibers and Enhanced Anticancer Effect in Combination with Ajwa-Dates-Extract (Phoenix dactylifera L.)
| Autor: | Adnan Memic, Kalamegam Gauthaman, Musab Aldhahri, Fazal Khan, Taha A. Kumosani, Rabah Wasil Aldhaheri, Nazeeh Alothmany, Elie K. Barbour, Adel M. Abuzenadah, Mohammad Asif Hussain |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemistry Atomic force microscopy technology industry and agriculture Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering macromolecular substances 02 engineering and technology 021001 nanoscience & nanotechnology In vitro Electrospinning 03 medical and health sciences PLGA chemistry.chemical_compound 030104 developmental biology Nanofiber Drug delivery General Materials Science Viability assay 0210 nano-technology Cytotoxicity Nuclear chemistry |
| Zdroj: | Journal of Biomedical Nanotechnology. 14:553-563 |
| ISSN: | 1550-7033 |
| Popis: | Side effects connected with chemotherapeutic agents used in cancer treatment has led to alternative modalities of combinatorial therapies in an attempt to reduce the drug dosage and associated risks. In the current study we evaluated the potential use of Ajwa Dates Extract (ADE), reported to have anti-cancer effects, as an adjuvant therapy in combination with 5-flurouracil (5FU) against the human-breast-adenocarcinoma cell line (MFC-7) in vitro. The effects of ADE alone and in combination with 5-FU were evaluated in terms of cell viability and cytotoxicity. For drug delivery purpose, we successfully encapsulated 5FU in both presence and absence of ADE through electrospinning together with poly lactic-co-glycolic acid (PLGA) in different combinations. Physicochemical properties of 5FU and ADE incorporated into PLGA nanofibers remained unaltered as confirmed by Fourier-Transform-Infrared (FTIR), Raman-spectroscopies and X-ray Diffraction (XRD) techniques. The morphological characterization of nanofibers was done using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The surface roughness of PLGA and PLGA + ADE nanofibers increased by incorporation of 5FU. PLGA + ADE nanofibers were in hydrophilic range ( |
| Databáze: | OpenAIRE |
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