Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

Autor:	Popy Hartatie Hardjo, Alfian Hendra Krisnawan
Rok vydání:	2020
Předmět:	Cefotaxime medicine.drug_class Antibiotics Benomyl Sterilization (microbiology) Biology Rhizome Fungicide chemistry.chemical_compound Horticulture chemistry medicine Zingiber officinale Explant culture medicine.drug
Zdroj:	MPI (Media Pharmaceutica Indonesiana). 3:19-26
ISSN:	2527-9017 2527-6298
DOI:	10.24123/mpi.v3i1.2836
Popis:	The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::31390e49be6756bd0951d9a843d99803 https://doi.org/10.24123/mpi.v3i1.2836 Zobrazit plný text záznamu