Survival and activity of lac-lux marked Pseudomonas aeruginosa UG2Lr cells encapsulated in κ-carrageenan over four years at 4°C
| Autor: | Hung Lee, M. B. Cassidy, Jack T. Trevors |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification biology Pseudomonas aeruginosa Pseudomonas Polysaccharide biology.organism_classification Shelf life medicine.disease_cause Microbiology Carrageenan chemistry.chemical_compound chemistry Pseudomonadales medicine Molecular Biology Bacteria Pseudomonadaceae |
| Zdroj: | Journal of Microbiological Methods. 30:167-170 |
| ISSN: | 0167-7012 |
| Popis: | Lac-lux marked Pseudomonas aeruginosa UG2Lr cells were encapsulated in alginate or a novel κ -carrageenan formulation. The beads were dried and maintained at 4°C in the dark. Plate counts determined that survival of encapsulated cells, after an initial Log 2 decrease over 24 h to Log 7.5 CFU g −1 dry beads during drying, only slightly decreased to Log 6.8 CFU g −1 dry beads after four years in storage. The lux activity of the genetically-modified bacteria was determined using a Biomedical Image Quantifier and all resulting colonies expressed luminescent activity. These results demonstrate the potential for long-term storage and subsequent use of encapsulated bacterial cells for environmental, as well as other applications. |
| Databáze: | OpenAIRE |
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