| Autor: |
Fridtjof Lund-Johansen, Nicolai Krogh, Brynjólfsdóttir Sh, Simon Bekker-Jensen, Steigerwald S, Jesper V. Olsen, Rasmus Kjøbsted, Trung Tran, Adi Mehta, Kwasniewicz E, Sikorski K, Alicia Lundby, Dorte B. Bekker-Jensen, Torres-Vega E, Lisa B. Frankel, Ana Martinez-Val |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry (MS)-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmarked the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro in HeLa cells and in-vivo in mouse tissues. Finally, we investigated the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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