Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+T cells
| Autor: | Paola Zanovello, Elisa Apolloni, Vincenzo Bronte, Paola Zamboni, Anna Cabrelle, Roberto Ronca, Paolo Serafini, Nicholas P. Restifo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Myeloid
Monocyte medicine.medical_treatment Immunology Antigen presentation Cell Biology Hematology Biology Biochemistry Molecular biology Granulocyte macrophage colony-stimulating factor medicine.anatomical_structure Cytokine medicine Cytotoxic T cell Antigen-presenting cell Interleukin 4 medicine.drug |
| Zdroj: | Blood. 96:3838-3846 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Apoptotic death of CD8+ T cells can be induced by a population of inhibitory myeloid cells that are double positive for the CD11b and Gr-1 markers. These cells are responsible for the immunosuppression observed in pathologies as dissimilar as tumor growth and overwhelming infections, or after immunization with viruses. The appearance of a CD11b+/Gr-1+ population of inhibitory macrophages (iMacs) could be attributed to high levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) in vivo. Deletion of iMacs in vitro or in vivo reversed the depression of CD8+ T-cell function. We isolated iMacs from the spleens of immunocompromised mice and found that these cells were positive for CD31, ER-MP20 (Ly-6C), and ER-MP58, markers characteristic of granulocyte/monocyte precursors. Importantly, although iMacs retained their inhibitory properties when cultured in vitro in standard medium, suppressive functions could be modulated by cytokine exposure. Whereas culture with the cytokine interleukin 4 (IL-4) increasediMac inhibitory activity, these cells could be differentiated into a nonadherent population of fully mature and highly activated dendritic cells when cultured in the presence of IL-4and GM-CSF. A common CD31+/CD11b+/Gr-1+ progenitor can thus give rise to cells capable of either activating or inhibiting the function of CD8+ T lymphocytes, depending on the cytokinemilieu that prevails during antigen-presenting cell maturation. |
| Databáze: | OpenAIRE |
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