MP66-12 THE EXPRESSION PROFILES OF MIR-210, MIR-375, MIR-378, MIR-345, MIR-143 MIR-183 AND MIR-98 IN THE PROGRESSION OF PROSTATE CANCER FROM HIGH-GRADE PROSTATIC INTRAEPITHELIAL NEOPLASIA TO METASTATIC DISEASES

Autor:	Fabian Woetzel, Ute Warnecke-Eberz, Axel Heidenreich, Hakan Alakus, Okyaz Eminaga, Jochen W.U. Fries
Rok vydání:	2016
Předmět:	PCA3 Oncology medicine.medical_specialty Matrigel business.industry Cell growth Urology Cell medicine.disease Metastasis Prostate cancer medicine.anatomical_structure Internal medicine LNCaP Cancer research Medicine High-grade prostatic intraepithelial neoplasia business
Zdroj:	Journal of Urology. 195
ISSN:	1527-3792 0022-5347
Popis:	was assessed by Western blot analysis and quantitative reverse transcription-PCR. To assess LASP1 function, we generated stable LASP1 knockdown and overexpression cell lines. For our cell growth assay, we used anchorage-independent growth conditions in 24 well plates coated with poly-Hema and monitored growth by WST-8 assay. To determine invasiveness, we monitored movement of cell through Matrigel coated invasion chambers. To measure tumorigenicity, in vivo tumor formation was assessed in mice xenografted with LNCaP cells overexpressing LASP1 RESULTS: Compared to low LASP1 expression, high LASP1 expression in prostate cancer is prone to early metastasis after radical prostatectomy (P1⁄40.0423). High LASP1 correlated with prostate cancer-specific mortality after radical prostatectomy (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::310f6d650cf897ab41b3b8e4e019d036 https://doi.org/10.1016/j.juro.2016.02.1286 Zobrazit plný text záznamu