HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF THYMOQUINONE IN RABBIT PLASMA AND ITS APPLICATION TO PHARMACOKINETICS

Autor:	Rao Muzaffar A. Khan, Ajaz Ahmad, Majed Al-Asmari, Khalid M. Alkharfy
Rok vydání:	2013
Předmět:	Detection limit Chromatography Clinical Biochemistry Extraction (chemistry) Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Pharmacokinetics Methanol Acetonitrile Thymol Thymoquinone
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 36:2242-2250
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826076.2012.717062
Popis:	The chemical compound thymoquinone (THQ) isolated from Nigella sativa seed oil has multiple therapeutic potentials. A simple and rapid reversed-phase high-performance liquid chromatography assay was developed to determine the concentration of THQ in rabbit plasma. The procedure involved extraction of the compound and thymol (internal standard) from the plasma using acetonitrile and methanol. The extract was injected onto Waters 5 µm Atlantis dC-18 Bond pack column (10 μ, 150 × 3.9 mm 1.d.). The mobile phase consisted of methanol and 10 mM KH2PO4 buffer in the ratio of 90:10 (v/v, pH = 4.6), which was delivered using isocratic flow at a rate of 0.9 mL/min. THQ and the internal standard were monitored using UV detection at 254 nm with a total run time of 7 min. The method was found to be linear (r 2 > 0.997), precise (CVs = 3.69–12.63%) and accurate (recovery = 88–107.77%) in the concentration range of 0.5–50 µg/mL. The limits of detection and quantitation of the method were 0.134 and 0.408 µg/mL, respecti...
Databáze:	OpenAIRE
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