A New Method for Total Protein Determination1

Autor:	W. J. Barreto, M. de Aquino, D. A. M. Zaia
Rok vydání:	1990
Předmět:	food.ingredient Chromatography Milk protein medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Biochemistry Analytical Chemistry Quinone food Casein Spectrophotometry Skimmed milk Electrochemistry medicine Kjeldahl method Spectroscopy Total protein
Zdroj:	Analytical Letters. 23:1279-1290
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032719008054353
Popis:	A new method for total protein determination was developed and applied to samples of skin milk powder. The method is based on the products of the reaction between p-benzoquinone and protein (casein), which show a charge-transfer band at 350 nm. The effects of concentration, reaction time, and pH were studied. The results were compared with those obtained with the Kjeldahl and Lowry methods; the standard deviations observed with the proposed method and with Lowry's method are of the same order of magnitude.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3103d6d22aff60a576ed2dafa27a99fb https://doi.org/10.1080/00032719008054353 Zobrazit plný text záznamu