Three Observations That Have Changed Our Understanding of Cyclin D1 and p27Kip1 in Cell Cycle Control
| Autor: | Dennis W. Stacey |
|---|---|
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Genes & Cancer. 1:1189-1199 |
| ISSN: | 1947-6027 1947-6019 |
| DOI: | 10.1177/1947601911403475 |
| Popis: | Our understanding of cell cycle control has been based largely upon studies of synchronized cultures, often focused upon the early stages of the cell cycle following stimulation of quiescent cultures. These studies showed that cyclin D1 and p27Kip1 (p27) each respond to the growth environment of the cell and together control entry into the cell cycle. In contrast, all cell cycle phases were considered in these studies of actively growing cultures, including events leading to withdrawal from the cell cycle. This approach relies upon the techniques of microinjection, quantitative image analysis, and time-lapse microscopy. The results provide critical new detail to our understanding of the roles of cyclin D1 and p27 in cell cycle regulation. Three critical observations resulting from this work will be described here to demonstrate that 1) cyclin D1 levels oscillate through the normal cell cycle, 2) checkpoint kinases are able to suppress cyclin D1 during S phase, and 3) the level of p27 is determined by a dy... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|