Diagnosis of Important Virus Diseases of Ornamental Anthurium in Screen- Houses in Tehran Province
| Autor: | Tabassom Ghotbi, Shahraeen Nooh |
|---|---|
| Rok vydání: | 2022 |
| Zdroj: | International Journal of Medical & Pharmaceutical Sciences. 12:01-06 |
| ISSN: | 2231-685X 2231-2188 |
| DOI: | 10.31782/ijmps.2022.12501 |
| Popis: | Introduction: One of the limiting factors for Anthurium production is its infection with various viruses. During the year 2014- 2015, a total of 504 symptomatic ornamental Anthurium leaf samples with virus-like symptoms including leaf and flower blister, dwarfing, mosaic, yellowing, leaf marginal chlorosis, and deformity were collected from Varamin and Pakdasht (Tehran province) Screen-houses. Aim: Samples were tested for infection to Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Dasheen mosaic viruses (DaMV). Method: Biological, serological (ELISA) and molecular methods (RT-PCR) were performed for the detection and analysis of the important viral agents infecting Anthurium ornamental plants. Results: According to ELISA test results the prevalence of CMV were (31.54%) followed by TSWV (25%) and DaMV (7.93%) viruses respectively. Results of mechanical inoculation and host range reaction of CMV, TSWV and DaMV isolates were also recorded (fig 2). Coat protein gene sequence of Iranian CMV isolate showed the highest similarity (97%) with a CMV isolate Cucurbit pepo (Acc. No.GU327368.1) from South Korea. DMV on ornamental Anthurium is reported for the first time in this research from Iran. Conclusion: Despite the detection of CMV, TSWV and DaMV in this study, viral pathogens are one of the most economically damaging agents in the ornamental Anthurium plant. Development of research methods for, the detection and study of different isolates of these agents from different hosts, and study of the characteristics of each of these isolates has always been a scientific need. |
| Databáze: | OpenAIRE |
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