Effects of autochthonous probiotics, isolated from Tor grypus (Karaman, 1971) intestine and Lactobacillus casei (PTCC 1608) on expression of immune-related genes
| Autor: | Takavar Mohammadian, Abdolhossein Jangaran Nejad, Esmaeil Karami, Mohammad Reza Tabandeh, Mojtaba Alishahi, Mojtaba Zarea |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lactobacillus casei biology food and beverages 04 agricultural and veterinary sciences Aquatic Science Antimicrobial biology.organism_classification In vitro 03 medical and health sciences Aeromonas hydrophila 030104 developmental biology Immune system Gene expression 040102 fisheries bacteria 0401 agriculture forestry and fisheries Food science Agronomy and Crop Science Bacteria Lactobacillus plantarum |
| Zdroj: | Aquaculture International. 27:239-260 |
| ISSN: | 1573-143X 0967-6120 |
| DOI: | 10.1007/s10499-018-0320-9 |
| Popis: | In this study, Lactobacillus plantarum subsp. plantarum and L. delbrukei subsp. bulgaricus were used as autochthonous food supplementation for gene expression and hematological investigation. Then, 480 fish (BW, 45 ± 10 g) were randomly divided into four groups (three replications) and were fed with diet containing 5 × 107 CFU g−1 of each isolated LAB and L. casei (PTCC 1608) and a control diet with no bacteria. Feeding trail was conducted for 60 days, following a change to the non-supplemented feed until 15 days (day 75). Samples from blood and head kidney at days 0, 60, and 75 were taken. The in vitro examination showed that L. plantarum had high antimicrobial activity against the Aeromonas hydrophila and Yesinia ruckeri. Results of this study showed that feeding of T. grypus with L. plantarum supplementation diet led to increase Hb and WBC, and induce TNF-α, IL-8, and IL-1β expressions in the head kidney rather than control. Results suggested that L. plantarum can be applied as a dietary supplement to enhance the immunity system of T. grypus. |
| Databáze: | OpenAIRE |
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