Toxicological and sanitary characteristics of fullerenol (Hydroxylated Fullerene C60) in 28-Day in vivo experiment
| Autor: | V. A. Shipelin, S. Yu. Batishcheva, Avren'eva Li, S Kh Soto, S. A. Khotimchenko, E N Trushina, Victor A. Tutelyan, I. V. Gmoshinski, G. Yu. Mal'tsev |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty biology Mean corpuscular hemoglobin concentration medicine.diagnostic_test Chemistry Glutathione reductase General Engineering CYP1A2 Cytochrome P450 Condensed Matter Physics chemistry.chemical_compound Endocrinology Biochemistry Internal medicine medicine biology.protein Uric acid General Materials Science In vivo experiment Glutathione transferase activity Polyunsaturated fatty acid |
| Zdroj: | Nanotechnologies in Russia. 8:799-809 |
| ISSN: | 1995-0799 1995-0780 |
| DOI: | 10.1134/s199507801306013x |
| Popis: | In a subacute toxicological and sanitary experiment, the hydroxylated fullerene C60, fullerenol C60(OH)24 is intragastrally administered to male Wistar rats at doses of 0.1, 1, and 10 mg/kg daily for 28 days. The integrated, biochemical, hematological, physiological, microbiological, and immunological indices that characterize the state of animals are determined. It is shown that a series of statistically reliable changes in indices occurs in rats during the daily administration of fullerenol at doses of 0.1 mg/kg and more for a month: a decrease in activity of cytochrome P450 CYP1A1 isoform at a dose of 0.1 mg/kg, a decrease in activity of cytochrome P450 CYP1A2 isoform at a dose of 0.1mg/kg and an increase in this parameter at 10 mg/kg, increased glutathione transferase activity at doses of 1 and 10 mg/kg, a decrease in the content of diene conjugates of polyunsaturated fatty acids (PUFAs) in plasma, and decreased activity of glutathione reductase in red blood cells at doses of 0.1 and 1 mg/kg. The level of uric acid, percentage content of monocytes, and relative content of immature granulocytes increases in a dose-dependent manner when fullerenol is administered at doses of 1 and 10 mg/kg, whereas the mean corpuscular hemoglobin concentration decreases within all ranges of the doses studied. Thus, based on these studies, we can conclude that the changes taking place in rats when fullerenol is administered at a dose of 0.1 mg/kg are small in absolute value and fall in the limits of the physiological norm variability. Consequently, it can be assumed that a fullerenol dose of less than 0.1 mg/kg may be considered maximally ineffective in a monthly experiment. |
| Databáze: | OpenAIRE |
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