3D-correlative FIB-milling and Cryo-ETof Autophagic structures in Yeast Cells v1

Autor:	Cristina Capitanio, Anna Bieber, Philipp S Erdmann, Brenda A Schulman, Wolfgang Baumeister, Florian Wilfling
Rok vydání:	2022
DOI:	10.17504/protocols.io.e6nvwkz4wvmk/v1
Popis:	This protocol describes how to plunge-freeze yeast on EM grids and how to target autophagic structures by combining cryo confocal fluorescence data to FIB-milling and tomogram acquisition
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::30b0e26e99b99f6a71b53f46dc5e2831 https://doi.org/10.17504/protocols.io.e6nvwkz4wvmk/v1 Zobrazit plný text záznamu