Direct fractionation of proteins in particle-containing feedstocks by a filter paper pieces-based DEAE-cellulose column chromatography

Autor:	Makoto Kawabata, Atsushi Sakata, Kozo Ohtsuki, Yasushi Nakamura, Yosuke Yamasaki, Jun Feng, Katsuo Komiya, Sumi Sugiyama, Yun-Hua Guo, Kenji Sato, Yutaka Minegishi, Masami Ichinomiya, Yasuyuki Tsukamasa
Rok vydání:	1998
Předmět:	Chromatography Filter paper Chemistry Pulp (paper) Organic Chemistry A protein General Medicine Fractionation engineering.material Biochemistry Analytical Chemistry Column chromatography engineering Particle Kiwi fruit DEAE-Cellulose
Zdroj:	Journal of Chromatography A. 811:69-76
ISSN:	0021-9673
Popis:	Filter paper pieces-based (FPB) DEAE-cellulose was prepared for direct fractionation of proteins in particle-containing feedstocks. FPB DEAE-cellulose has a protein binding capacity equivalent to that of commercially available DEAE-cellulose. Crude extracts from porcine intestine and kiwi fruit pulp, which were unmanageable by commercially available chromatographic media due to rapid clotting, could be directly fractionated with FPB DEAE-cellulose column. In addition, effluents from an FPB DEAE-cellulose column were extensively clarified. The present approach can be used as a rapid, robust and low-cost capturing step for protein from particle-containing feedstocks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3089be72f9a93a756b4134ed32c0b641 https://doi.org/10.1016/s0021-9673(98)00251-9 Zobrazit plný text záznamu Full Text from ScienceDirect