Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay

Autor: Alessio Valentini, Wouter Coppieters, Luc Grobet, Michel Georges, Latifa Karim
Rok vydání: 2000
Předmět:
Zdroj: Animal Genetics. 31:396-399
ISSN: 0268-9146
DOI: 10.1046/j.1365-2052.2000.00684.x
Popis: We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
Databáze: OpenAIRE