Viral Protein Synthesis by Tissues from Avian Leukosis Virus-Infected Chickens
| Autor: | S. Welt, H. G. Purchase, G. J. Thorbecke |
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| Rok vydání: | 1977 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 119:1800-1805 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.119.5.1800 |
| Popis: | Chickens of susceptible line 15I were infected at hatching with avian leukosis virus. Viral protein synthesis was detected by showing 14C amino acid incorporation into these proteins by tissues in vitro as shown by autoradiography of viral group-specific (gs) protein immunoelectrophoretic patterns. Synthesis of viral gs proteins p27, p15, and p12 was first detected in all tissues examined of 18-day-old chickens (thymus, spleen, bursa, liver, kidney, testis, ovary, and lung), continued up to 7 to 9 weeks, but could not be detected at 10 weeks, except in lung and genital organs. Grossly leukotic chickens showed gs protein synthesis primarily in the bursa and spleen but, depending on the degree of leukotic infiltration, also in the liver and kidney. Tissues from uninfected controls never showed any gs protein synthesis. The results indicated that all tissues tested were active in viral replication during the initial viremic stage of the disease, whereas during the leukotic stage, lymphoma cells were the predominant cell type active in viral protein synthesis. Enhanced IgG synthesis in spleen tissue from infected chickens during the viremic stage suggested the presence of a humoral immune response to the virus. Leukotic tissue also appeared to produce both IgM and IgG. |
| Databáze: | OpenAIRE |
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