Pilot plant isolation of transaldolase fromCandida utilis
Autor: | C. C. Matteo, O. Tsolas, D. Wallace, H. E. Blair, Stanley E. Charm |
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Rok vydání: | 1970 |
Předmět: | |
Zdroj: | Biotechnology and Bioengineering. 12:321-331 |
ISSN: | 1097-0290 0006-3592 |
DOI: | 10.1002/bit.260120211 |
Popis: | Through the use of pilot plant equipment, transaldolase types I, II, and III (from Candida utilis) have been separated and purified. The procedure includes a time sensitive solvent fractionation below 0°C, ion exchange chromatography, and crystalization. The enzyme yield represents a 41% recovery of crystalline type III and partially purified types I and II. |
Databáze: | OpenAIRE |
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