Novel Microsatellite Development and Characterization for Phacelia formosula (Hydrophyllaceae)
| Autor: | Anna L. Schwabe, Jennifer Ramp Neale, James P. Riser |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Conservation genetics Genetics Genetic diversity biology Locus (genetics) Plant Science biology.organism_classification 010603 evolutionary biology 01 natural sciences Gene flow Loss of heterozygosity 03 medical and health sciences 030104 developmental biology Evolutionary biology Phacelia Genetic variation Microsatellite Ecology Evolution Behavior and Systematics |
| Zdroj: | Applications in Plant Sciences. 5:1700030 |
| ISSN: | 2168-0450 |
| DOI: | 10.3732/apps.1700030 |
| Popis: | Premise of the study: Microsatellite primers were developed to characterize genetic diversity and structuring in the genus Phacelia (Hydrophyllaceae) and to further conservation efforts for P. formosula. Methods and Results: Fifteen novel microsatellite primers were developed for P. formosula. These were characterized for genetic variation in three separate P. formosula populations. Two to nine alleles were found per locus. Overall observed heterozygosity and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Additionally, these loci were successfully amplified and showed polymorphism in P. gina-glenneae and a potential new Phacelia species. Conclusions: These microsatellite markers will be useful in assessing genetic diversity, structuring, and gene flow within and among populations of the rare P. formosula, in addition to related Phacelia species. These markers will provide important genetic data needed for appropriate conservation and management of these rare plants. |
| Databáze: | OpenAIRE |
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