Use of Enzyme-Linked Antibodies to Measure Serum Anti-DNA Antibody in Systemic Lupus Erythematosus

Autor:	N. Mendoza, Victor E. Pollak, M. A. Gaizutis, Amadeo J. Pesce, Irena G. Boreisha
Rok vydání:	1974
Předmět:	chemistry.chemical_classification medicine.diagnostic_test biology Chemistry Biochemistry (medical) Clinical Biochemistry Substrate (chemistry) Molecular biology Anti-DNA Antibody chemistry.chemical_compound Enzyme Biochemistry Immunoassay medicine biology.protein Antibody DNA Peroxidase Conjugate
Zdroj:	Clinical Chemistry. 20:353-359
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/20.3.353
Popis:	We describe a solid-phase adsorbent enzyme-linked immunoassay for measuring the amount of antibody in serum. The assay consists of adsorbing an excess of DNA to a polystyrene test-tube support, reacting the anti-DNA serum with the coated tube, following with an additional reaction of an anti-human γ-globulin peroxidase enzyme conjugate with the coated tube, and finally developing a colored reaction product by the action of peroxidase on substrate. In principle, the amount of IgG bound to the tube is proportional to the amount of anti-DNA contained in the serum, and the conjugate also reacts proportionally to the amount of human IgG that has reacted. The assay gives data consistent with that expected for serum from patients with systemic lupus erythematosus, and it is possible to titrate the serum for micrograms of DNA antibody. At present the method still has considerable variability, but offers a new approach to measurement of antibody in autoimmune diseases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::305fe229c0a05cfd8a6ad6f6e8dac61f https://doi.org/10.1093/clinchem/20.3.353 Zobrazit plný text záznamu