Differential polarization microscope using an image dissector camera and phase‐lock detection

Autor:	Carlos Bustamante, David Beach, K. S. Wells, F. Husher
Rok vydání:	1987
Předmět:	Image dissector Microscope Materials science Photoelastic modulator business.industry Amplifier Optical polarization Polarization (waves) law.invention Optics Optical microscope law Demodulation business Instrumentation
Zdroj:	Review of Scientific Instruments. 58:1987-1995
ISSN:	1089-7623 0034-6748
DOI:	10.1063/1.1139505
Popis:	A polarization modulation microscope designed to detect and spatially resolve the distribution of optically anisotropic domains in microscopic objects is described. An image dissector camera is used as the detection device. The polarization of the light is modulated by means of a photoelastic modulator and a synchronous demodulation amplifier (lock‐in) is utilized to obtain the difference image between the images formed using two orthogonal forms of the incident polarization on the sample. Preliminary results are described for the linear dichroism imaging measurements of polymerized hemoglobin (HbS) in red blood sickle cells. The present limitations of this design are discussed along with various strategies to improve its performance.
Databáze:	OpenAIRE
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