Characterization of the photoautotrophic algal and bacterial communities in a large, shallow, subtropical lake using HPLC-PDA based pigment analysis

Autor:	J. William Louda, Earl W. Baker, Nancy M. Winfree, Karl E. Havens, Alan D. Steinman
Rok vydání:	1998
Předmět:	Pheophytin Chlorophyll a Sediment Photosynthetic pigment Aquatic Science Biology Anoxygenic photosynthesis chemistry.chemical_compound Water column chemistry Environmental chemistry Botany Phytoplankton Fucoxanthin Ecology Evolution Behavior and Systematics
Zdroj:	Canadian Journal of Fisheries and Aquatic Sciences. 55:206-219
ISSN:	1205-7533 0706-652X
DOI:	10.1139/f97-239
Popis:	Pigment abundances of the oxygenic and anoxygenic photoautotrophic communities from sediments and the water column in Lake Okeechobee, Florida, were estimated using reverse-phase high-performance liquid chromatographic (RP-HPLC) and photodiode array (PDA) UV/VIS (350-800 nm) spectrophotometric analyses. Thirty lipophilic pigments were identified and measured in the samples, with the most abundant overall (sediment and open-water samples combined) being chlorophyll a (38.1%), fucoxanthin (12.6%), pheophytin a (7.6%), zeaxanthin (6.6%), and pyropheophytin a (3.6%). Relative abundance of chlorophyll a was greater in the water column than in the sediments (58.3 versus 24.3% of all pigments) whereas pheophytin a comprised 9.1% of the total pigments in the sediments but only 3.7% of the total pigments in the water column. Principal component analysis (PCA) separated the sediment samples from those collected in the water column; this discrimination appears to be a function of pigment integrity in that sediment assemblages had much greater relative abundances of degraded pigments. Different regions of the lake were weakly separated by PCA based on pigments. The relatively weak degree of separation may reflect the overwhelming abundance of chlorophyll a at all sites. Overall, the pigment assemblage in Lake Okeechobee suggests cyanobacteria-diatom dominance. Out of 65 sampling events, pigments from anoxygenic photoautotrophs (e.g., bacteriochlorophylls) were detected 17 times but accounted for >20% of total chlorophyll only five times. Bacteriochlorophylls were observed only in the sediments and were most abundant during June and September, when winds were calm and temperatures warm, and at relatively shallow sites.
Databáze:	OpenAIRE
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