Isolation of Two Cell Lines from a Human Malignant Glioma Specimen Differing in Sensitivity to Radiation and Chemotherapeutic Drugs
| Autor: | K. D. Dobler, M. J. Allalunis-Turner, Rufus S. Day, Razmik Mirzayans, G. M. Barron |
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| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty Radiation Radiobiology Biophysics Biology medicine.disease Nitrogen mustard Ionizing radiation chemistry.chemical_compound Radiation sensitivity chemistry Cell culture Glioma medicine Cancer research Cytotoxic T cell Radiology Nuclear Medicine and imaging Radiosensitivity |
| Zdroj: | Radiation Research. 134:349 |
| ISSN: | 0033-7587 |
| DOI: | 10.2307/3578196 |
| Popis: | Two aneuploid cell lines which differ in their inherent sensitivity to ionizing radiation and chemotherapeutic agents were established concurrently from a single tumor specimen obtained from a patient with glioblastoma. M059J cells are approximately 30-fold more sensitive to radiation than are M059K cells (surviving fractions at 2 Gy were 0.02 and 0.64, respectively). This relative difference in radiation sensitivity has remained a stable feature of the cell lines during 2 years in continuous culture. In addition, cells of the M059J line are more sensitive than those of the M059K line to the cytotoxic effects of bleomycin, N,N-bis(2-chloroethyl)-N-nitrosourea, and nitrogen mustard. These cell lines may prove to provide a useful model system for evaluating the cellular and molecular processes which confer resistance or sensitivity in cancer treatment. |
| Databáze: | OpenAIRE |
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