Popis: |
Benzoate, dimethylsulphoxide, mannitol and methionine, which are scavengers of the hydroxyl radical (OH·), inhibited the accumulation of the glyceollin phytoalexins in soybean cotyledons elicited by silver nitrate. Benzoate also inhibited phytoalexin accumulation in groundnut cotyledons elicited by slicing and in pea cotyledons elicited by slicing or silver nitrate. Superoxide, generated by the xanthine oxidase/acetaldehyde reaction, did not cause glyceollin accumulation in soybean cotyledons and superoxide dismutase did not prevent glyceollin accumulation by silver nitrate treatment. These results are discussed in terms of a direct role for OH· but not superoxide in the process of abiotic elicitation of phytoalexins in legume cotyledons. |