Development of a loop-mediated isothermal amplification assay for detecting Listeria monocytogenes prfA in milk
| Autor: | Ae-Ri Cho, Hee-Jin Dong, Seongbeom Cho, Kun-Ho Seo |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Detection limit
biology Enrichment broth Chemistry Pcr assay Loop-mediated isothermal amplification biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology eye diseases Listeria monocytogenes Listeria medicine Screening tool Incubation Food Science Biotechnology |
| Zdroj: | Food Science and Biotechnology. 23:467-474 |
| ISSN: | 2092-6456 1226-7708 |
| DOI: | 10.1007/s10068-014-0064-x |
| Popis: | A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed for detecting Listeria monocytogenes prfA in milk. The inclusivity of 23 L. monocytogenes and the exclusivity of 16 non-L. monocytogenes strains were both 100% in the assay. The limit of detection (LoD) of the LAMP assay in Listeria enrichment broth (LEB) was 2.22 CFU/mL after 12 h and 24 h of incubation. The LoDs of the LAMP assay in LEB with artificially contaminated milk (LEB-M) incubated for 12 h (2.22×101 CFU/mL) and 24 h (2.22 CFU/mL) were lower than those of the PCR and real-time PCR assays. Comparison of the LoDs in LEB with those in LEB-M showed that the LAMP assay was less influenced by the milk compounds than the real-time PCR assay. Our results indicate that the LAMP assay can be utilized as a potential screening tool for L. monocytogenes in milk. |
| Databáze: | OpenAIRE |
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