Effect of fsDNA on chemiluminescence characteristics of luminol–H2O2 system catalyzed by Mn(III)–Tetrakis (4-sulfonatophenyl)-porphyrin

Autor: Seyed Mohammad Abedirad, Sayed Yahya Kazemi
Rok vydání: 2012
Předmět:
Zdroj: Journal of the Iranian Chemical Society. 10:251-256
ISSN: 1735-2428
1735-207X
DOI: 10.1007/s13738-012-0153-9
Popis: In the present work, the effect of fsDNA (fish sperm DNA) on kinetic parameters of chemiluminescence (CL) of the luminol–hydrogen peroxide system catalyzed by Mn(III)–Tetrakis (4-sulfonatophenyl)-porphyrin was investigated. These parameters including pseudo first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL were evaluated by computer fitting of the resulted intensity–time plots. Results reveal that CL parameters are dramatically affected due to interaction of metalloporphyrin with DNA. In order to observe quenching effect of DNA on CL intensity, Stern–Volmer plot with kQ value of 1.18 × 105 M−1 was calculated in the quencher concentration range of 4 × 10−6–8.5 × 10−5 M.
Databáze: OpenAIRE
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