Topical application of Azadirachta indica improves epidermal wound healing in hyperglycemic rats
| Autor: | João Vitor Silvano Bittencourt, Andrea Aparecida de Aro, Laura de Roch Casagrande, Fernanda Oliveira de Gaspari de Gaspi, Ana C. C. Silva, Samara de Sousa Mariano, Gláucia Maria Tech dos Santos, Paulo Cesar Lock Silveira, Sofia Poletti, Marcelo Augusto Marreto Esquisatto, Thiago Antônio Moretti de Andrade, Aline N. Eugênio |
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| Rok vydání: | 2021 |
| Předmět: |
integumentary system
biology 040301 veterinary sciences Chemistry food and beverages 04 agricultural and veterinary sciences Glutathione Pharmacology Azadirachta biology.organism_classification medicine.disease_cause Pathology and Forensic Medicine Nitric oxide 0403 veterinary science Superoxide dismutase chemistry.chemical_compound Myeloperoxidase biology.protein Poor wound healing medicine Anatomy Wound healing Oxidative stress |
| Zdroj: | Comparative Clinical Pathology. 30:461-472 |
| ISSN: | 1618-565X 1618-5641 |
| DOI: | 10.1007/s00580-021-03240-1 |
| Popis: | Diabetes mellitus, which is characterized by hyperglycemia, leads to poor wound healing. Azadirachta indica has antidiabetic properties and further promotes the healing of epidermal wounds. Here, we investigated the effects of topical application of A. indica extract on excisional lesions in hyperglycemic rats. The animals were divided into four groups (n=15): non-hyperglycemic and treated with carbopol gel (NH), non-hyperglycemic and treated with A. indica + carbopol gel (NHT), hyperglycemic and treated with carbopol gel (H), and hyperglycemic and treated with A. indica + carbopol gel (HT). Lesions were made on the animals’ back, with a punch of 1.5-cm diameter. Samples of the skin/lesion were collected on the 2nd, 7th, and 14th days post-injury for histological examination (inflammatory infiltrate, blood vessels, and fibroblasts), biochemical analysis (MPO [myeloperoxidase], neutrophilic infiltrate and NAG [N-acetylglycosaminidase], and macrophagic infiltrate), oxidative stress (oxidants such as NO [nitric oxide] and DCF [2′,7′-dichlorodihydrofluorescein diacetate], oxidative damage by carbonyl sulfhydryl radicals, and antioxidants such as SOD [superoxide dismutase] and GSH [glutathione]), and analysis of reepithelialization by assessing the wound healing rate. The topical application of A. indica extract in the excisional wounds of hyperglycemic rats accelerated wound healing through anti-inflammatory, antioxidant, angiogenic, and fibrillogenic effects. Thus, these results show that A. indica extract has the potential to be used as a therapeutic agent for treating excisional wounds in hyperglycemic. |
| Databáze: | OpenAIRE |
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