Changes in the crystal morphology, chemical composition and key gene expression of 'Suichuan' kumquat cuticular waxes after hot water dipping

Autor: Li Yang, Wei Hu, Li Qiu, Yong Liu, Dechun Liu, Liuqing Kuang
Rok vydání: 2022
Předmět:
Zdroj: Scientia Horticulturae. 293:110753
ISSN: 0304-4238
DOI: 10.1016/j.scienta.2021.110753
Popis: The aim of this paper was to identify changes in the crystal morphology, chemical composition and key gene expression of 'Suichuan' kumquat cuticular waxes after hot water dipping treatment (HWD), before and after cold storage, as well as the relationship of cuticular waxes with storage performance improvement. Scanning electron microscopy (SEM) showed a rearrangement of epicuticular wax layers after HWD treatment, and the layers did not crack at 30 d of storage, which is opposite the results of control fruit, which might be related to the reduction in water loss and decay rate by covering the cracked wax and any wax deficiencies. Twenty-eight identified constituents that could be classified into six categories in cuticular wax were observed in both HWD-treated and control fruit before and after storage. Triterpenoids accounted for the largest proportion of total waxes, followed by fatty acids, aldehydes, alkanes, primary alcohols, and stigmasterol. The amount of total wax and triterpenoids in HWD-treated fruit was lower than that of the control group during storage, and the opposite results were observed for the relative proportion of aldehydes or alkanes and the ratio of aldehydes or alkanes to triterpenoids during storage. Therefore, we speculated that the changes in alkanes, aldehydes and triterpenoids, rather than the total wax, might be the key factors improving the storage performance after HWD. The expression level of the CER1 gene involved in the accumulation of alkanes and the LUP2 and LUP4 genes involved in the accumulation of triterpenoids were positively related to the changes in the contents of corresponding cuticular wax components during storage. These results shed new light on the chemical and molecular basis for wax involvement in storage after HWD treatment.
Databáze: OpenAIRE