Cytosolic glutamine synthetase (GS1): posttranslational regulation and role in nitrogen remobilization from leaves of non-transgenic and GS1 -over-expressing oilseed rape plants
| Autor: | J. K. Schjoerring, J. Finnemann, C. Möllers |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0106 biological sciences
0303 health sciences Kinase Phosphatase food and beverages Biology 01 natural sciences Serine 03 medical and health sciences Biochemistry Glutamine synthetase Phosphorylation Post-translational regulation Threonine Protein kinase A 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Plant Nutrition ISBN: 9780792371052 |
| DOI: | 10.1007/0-306-47624-x_57 |
| Popis: | Expression and immune-detection studies showed that cytosolic glutamine synthetase (GS1) is the only active GS isozyme in senescing leaves of B. napus L. and that GS1 is a phospho-protein, which is regulated post-translationally by reversible phosphorylation catalysed by protein kinases and microcystin-sensitive serine/threonine protein phosphatases. Dephosphorylated GS1 is much more susceptible to degradation than the phosphorylated form. Phosphorylated GS1 can interact with 14-3-3 proteins. The degree of interaction with 14-3-3-proteins can in vitro be modified by decreasing or increasing the phosphorylation status of GS1 Transgenic oilseed rape plants overexpressing GS1 (2–3 fold higher GS1 activity in senescing leaves compared to wild-type plants) had a more efficient remobilization of N. |
| Databáze: | OpenAIRE |
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