Determination of pyrethroid metabolites in human urine by capillary gas chromatography-mass spectrometry
| Autor: | K. A. Bucholski, H. Idel, K. H. Kühn, L. Dunemann, G. Leng |
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| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Chromatography Carboxylic acid Metabolite Organic Chemistry Clinical Biochemistry Urine Cyfluthrin Biochemistry Analytical Chemistry chemistry.chemical_compound Hydrolysis chemistry Gas chromatography Gas chromatography–mass spectrometry Quantitative analysis (chemistry) |
| Zdroj: | Chromatographia. 43:285-292 |
| ISSN: | 1612-1112 0009-5893 |
| Popis: | An analytical method for the simultaneous determination of the pyrethroid metabolites cis and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid, cis 3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane carboxylic acid, 3-phenoxybenzoic acid and 4-fluoro-3-phenoxybenzoic acid in human urine samples is described. The urine is subjected to acid-induced hydrolysis followed by exhaustive solvent extraction, covering both conjugated and free acids, followed by a common derivatisation step yielding the corresponding methyl esters. Quantitation was by diastereomeric, capillary gas chromatography-mass spectrometry. It appears that 4-fluoro-3-phenoxybenzoic acid is a characteristic urinary marker for cyfluthrin exposure. The limits of determination are 0.5–1.0 μg L−1 urine depending on the metabolites concerned. The applicability of the method was tested on urine samples from pest control operators exposed occupationally to cypermethrin and cyfluthrin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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