CRISPR/Cas9 Systems: The Next Generation Gene Targeted Editing Tool

Autor:	Yuxiang Lin, Shengchun Guo, Ying Lv, Zhining Li, Peng Peng, Jingli Peng, Shaoyun Song, Yangxiu Wu, Qiuyun Liu, Kaixuan Lin
Rok vydání:	2014
Předmět:	Genetics CRISPR-Associated Proteins Nucleic acid chemistry Genome editing Cas9 RNA CRISPR Biology General Agricultural and Biological Sciences Gene General Environmental Science Genome engineering
Zdroj:	Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 85:377-387
ISSN:	2250-1746 0369-8211
DOI:	10.1007/s40011-014-0362-3
Popis:	Type II CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) system based on RNA and Cas9 protein is an essential part of adaptive immune systems of bacteria and archaea against invading genetic elements. Both the RNA elements and the protein component of the system can be reprogrammed, making it a powerful and versatile tool for genome engineering in various types of organisms. Compared with other tools in genome editing, the CRISPR/Cas system is characterized by its specificity, simplicity and diversity, and yet the procedure is rapid and cost effective. This review presents the latest developments and methodologies on the applications of Type II CRISPR/Cas system in gene targeted editing. Future prospects of the technology are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2fb8efca77f655f1851a049eee24087e https://doi.org/10.1007/s40011-014-0362-3 Zobrazit plný text záznamu Full text from SpringerLink