Detection of human polyomaviruses MCPyV, HPyV6, and HPyV7 in malignant and non-malignant tonsillar tissues
| Autor: | Zuzana Vojtěchová, Vojtěch Šroller, Martina Salakova, Eva Koslabova, Ruth Tachezy |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
education.field_of_study Pathology medicine.medical_specialty biology Merkel cell carcinoma Population Merkel cell polyomavirus Non malignant medicine.disease Malignancy biology.organism_classification Virology Serology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Infectious Diseases chemistry medicine education Viral load DNA |
| Zdroj: | Journal of Medical Virology. 88:695-702 |
| ISSN: | 0146-6615 |
| Popis: | Merkel cell polyomavirus (MCPyV) is associated with Merkel cell carcinoma (MCC), a rare skin malignancy. Human polyomavirus six and seven (HPyV6 and HPyV7) were identified on a skin but have not been associated with any pathology. The serology data suggest that infection with polyomaviruses occurs in childhood and they are widespread in population. However, the site of persistent infection has not been identified. Altogether, 103 formalin-fixed paraffin-embedded (FFPE) specimens and five fresh frozen tissues (FF) of non-malignant tonsils and 97 FFPE and 15 FF samples of tonsillar carcinomas were analyzed by qPCR for the presence of MCPyV, HPyV6, and HPyV7 DNA. All MCPyV DNA positive FF tissues were screened for the expression of early viral transcripts. Overall prevalence of MCPyV, HPyV6, and HPyV7 in non-malignant tonsillar tissues was 10.2%, 4.6%, and, 0.9%, respectively. The prevalence of MCPyV DNA in non-malignant tonsils increased with age (P |
| Databáze: | OpenAIRE |
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