Metrological support for DNA sequencing

Autor:	A. V. Mardanov, N. V. Ravin, S. A. Kononogov, Yu. A. Kudeyarov, S. S. Golubev, P. Yu. Nikolaeva, Konstantin G. Skryabin
Rok vydání:	2012
Předmět:	DNA nanoball sequencing Computer science Shotgun sequencing Applied Mathematics Nucleic acid sequence Pyrosequencing Ion semiconductor sequencing Computational biology Instrumentation DNA sequencing Illumina dye sequencing Sequencing by ligation
Zdroj:	Measurement Techniques. 55:345-350
ISSN:	1573-8906 0543-1972
DOI:	10.1007/s11018-012-9962-6
Popis:	We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2fa039ad77379dbcb1a42dbabad4d621 https://doi.org/10.1007/s11018-012-9962-6 Zobrazit plný text záznamu Full text from SpringerLink