Antibody Plaque-Forming Cells in Mice Immunized with Human γ Globulin

Autor:	John S. Hege, Wallace V. Epstein
Rok vydání:	1971
Předmět:	Immunology Immunology and Allergy
Zdroj:	The Journal of Immunology. 106:786-792
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.106.3.786
Popis:	In order to increase the efficiency and reliability of the carbodiimide modification of the Jerne plaque technique several variables were analyzed. Spleen cells forming antibody directed against human γ globulin (HGG) were enumerated in mice undergoing primary or secondary responses to immunization with this protein. When the Jerne indicator erythrocytes were coated with heat-aggregated HGG rather than native HGG the numbers of plaques detected among spleen cells from mice undergoing primary responses were reduced. Small degrees of HGG aggregation caused by freezing and thawing did not affect plaque formation. An optimal concentration of complement was determined for the plaque assay procedure which resulted in a marked increase in plaquing efficiency. The numbers of plaques determined as a function of time after primary HGG immunization reached a maximum on the 6th day.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2f9f5746cac884bd8ba74d4f71719ac3 https://doi.org/10.4049/jimmunol.106.3.786 Zobrazit plný text záznamu