| Popis: |
Publisher Summary In this chapter, procedures for the modulation by β-carotene of the delayed light emission (DLE) from aggregated chlorophyll (Chl) and detection of energy storage and transfer between carotene and Chl are given. DLE has been observed in a suspension of aggregated Chl. It was shown that the intensity and decay rate of DLE from aggregated Chl depend on several factors, such as the intensity and duration of illumination, the presence of electron donors and acceptors, and the presence of deaggregating reagents. Of special interest is the fact that not only do unilluminated pheophytin, Chlb, and carotenoids have effects on the DLE from aggregated Chla, but the illuminated pigments can also induce DLE from unilluminated aggregated Chla, although no DLE can be detected after illumination. These phenomena can be used to probe energy storage and transfer between aggregated Chl and other photosynthetic pigments. By injecting solutions of carotene having various concentrations, illuminated at different light intensities, and for different durations into cuvettes containing aggregated Chl in the dark chamber, a group of DLE curves is obtained. |
