Coffea arabica L. genes from isoprenoid metabolic pathways are more expressed in full sun cultivation systems than in agroforestry systems
| Autor: | Suzana Tiemi Ivamoto-Suzuki, Fernanda Freitas de Oliveira, Tiago Benedito dos Santos, Bruna Silvestre Rodrigues da Silva, Maria Brígida dos Santos Scholz, Luiz Filipe Protasio Pereira, Juarez Pires Tomaz |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agroforestry Coffea arabica Cafestol Plant Science Biology 01 natural sciences Biochemistry Terpenoid Crop 03 medical and health sciences Metabolic pathway chemistry.chemical_compound 030104 developmental biology Nutraceutical chemistry Genetics medicine Plant defense against herbivory 010606 plant biology & botany Biotechnology medicine.drug Kahweol |
| Zdroj: | Plant Gene. 26:100287 |
| ISSN: | 2352-4073 |
| Popis: | Coffee synthesizes two unique isoprenoid diterpenes, cafestol (C20H28O3) and kahweol (C20H26O3), ent-kaurene compounds produced through the mevalonate or mevalonate-independent pathway. These compounds are relevant to beverage quality, plant defense, human health and nutraceutical properties. We investigate the expression profile of genes in the early (CaHMGR, CaMVD, CaDXR and CaIDS) and final steps of isoprenoid diterpenes biosynthesis (Coffea arabica L. cytochrome P450s: CaCYP82C2, CaCYP71A25, CaCYP74A1 and CaCYP701A3) in two fruits development stages (90 and 210 days after flowering). We also observed total lipid, cafestol and kahweol accumulation in C. arabica grown using two crop systems (full sun and agroforestry) at IDR-Parana Londrina, PR, Brazil. Plants in full sun presented higher gene expression than plants grown in agroforestry systems, except for CaHMGR. Fruits showed higher levels of transcript activity 210 days after flowering, about 2.8 times more in the genes expressed during the first steps and 3.6 times in those in the later steps. Although lipid, cafestol and kahweol were higher in the full sun than the agroforestry system, only increased lipid content was statistically significant. Cultivation system plays an important role in the expression of the genes, particularly as regards light incidence. |
| Databáze: | OpenAIRE |
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