Epithelial tissue formation in matrix culture and in histophysiologic gradient culture

Autor:	Joseph Leighton
Rok vydání:	1992
Předmět:	Radial gradient Cell Biology Anatomy Biology Matrix (biology) biology.organism_classification Epithelium Pathology and Forensic Medicine Cell biology Sponge Tissue culture medicine.anatomical_structure medicine Epithelial tissue
Zdroj:	Journal of Tissue Culture Methods. 14:201-207
ISSN:	1573-0603 0271-8057
DOI:	10.1007/bf01409012
Popis:	Formation of epithelial tissues in culture so that they become facsimiles in their structure of such tissues in nature requires procedures that comply with several spatial imperatives: a) three-dimensional growth; b) histophysiologic conditions that provide, concurrently, gradients of maturation and of diffusion of metabolites; and c) growth as layers of cells without free edges. Many steps have been required in the evolution of these methods. Two systems are described here in sufficient detail to serve as a manual. Three-dimensional growth of masses of epithelial tissue is accomplished in matrix culture using Gelfoam sponge and collagen-coated cellulose sponge. Radial gradient culture, a recent development, provides conditions that comply with the requirements of histophysiologic gradients and of epithelial tissue growth in layers without interruption in their continuity.
Databáze:	OpenAIRE
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