Structure of arabinogalactan from Larix laricina and its reactivity with antibodies directed against type-II-arabinogalactans

Autor: Birgit Classen, Jon Utermoehlen, E Goellner, Rainer Kramer
Rok vydání: 2011
Předmět:
Zdroj: Carbohydrate Polymers. 86:1739-1744
ISSN: 0144-8617
Popis: An arabinogalactan from wood of the larch tree ( Larix laricina ), which is a source of dietary fiber, is composed of d -galactose and l -arabinose in a 6:1 molar ratio accompanied by small amounts of d -glucuronic acid. The molecular mass has been determined to be 38 kDa. Linkage analysis in combination with partial hydrolysis and spectroscopic investigations revealed a 1,3-linked Gal p backbone, branched at C6 to 1,6-linked Gal p side residues terminated by Gal p , Ara p , Ara f or Glc p A. Monoclonal antibodies directed against a type-II-arabinogalactan from Echinacea purpurea showed cross reactivity with the larch arabinogalactan and have been used to develop an ELISA for quantification of this polysaccharide.
Databáze: OpenAIRE