| Autor: |
H. Xu, Q. Kong, M. Xiao, F. Kong, H. Xiang, X. Ni, H. Jin, L. Shen, M. Brown, Jeong-Ok Cha, J. Sun, L. Wei |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of Applied Microbiology. 111:625-630 |
| ISSN: |
1364-5072 |
| Popis: |
Aims: For the rapid detection of Laribacter hongkongensis, which is associated with human community-acquired gastroenteritis and traveller’s diarrhoea, we developed a duplex species-specific PCR assay. Methods and Results: Full-length of the 16S–23S rRNA intergenic spacer region (ISR) sequences of 52 L. hongkongensis isolates were obtained by PCR-based sequencing. Two species-specific primer pairs targeting 16S rRNA gene and ISR were designed for duplex PCR detection of L. hongkongensis. The L. hongkongensis species-specific duplex PCR assay showed 100% specificity, and the minimum detectable level was 2·1 × 10−2 ng μl−1 genomic DNA which corresponds to 5000 CFU ml−1. Conclusions: The high specificity and sensitivity of the assay make it suitable for rapid detection of L. hongkongensis. Significance and Impact of the Study: This species-specific duplex PCR method provides a rapid, simple, and reliable alternative to conventional methods to identify L. hongkongensis and may have applications in both clinical and environmental microbiology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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