Detection of Human Papillomaviruses DNA in paired peripheral blood and cervix samples patients with cervical lesions and healthy individuals
| Autor: | Antônio Roberto Lucena, Marconi Rego Barros Júnior, Marcus Vinicius de Aragão Batista, Ana Pavla Almeida Diniz Gurgel, Antonio Carlos de Freitas, Elyda Golçalves de Lima, Bárbara Simas Chagas, Zhilbelly da Mota Nunes, Kamylla Conceição Gomes Nascimento, Jacinto da Costa Silva Neto |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Hpv types business.industry virus diseases Cervical intraepithelial neoplasia medicine.disease Gastroenterology female genital diseases and pregnancy complications Peripheral blood chemistry.chemical_compound medicine.anatomical_structure chemistry Healthy individuals Internal medicine medicine business Viral load Cervix Gene DNA |
| DOI: | 10.1101/2021.05.29.20161125 |
| Popis: | This study evaluated the presence of HPV DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (n= 68) were tested for HPV DNA by using standard procedures. PCR-sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8-55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1-77.7% in CIN patients), and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5-44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (P=0.023; OR: 2.978; CI:1.34-7.821), HPV DNA in the blood (P=0.000; OR: 3.369; CI:3.700-18.540), and cervical lesions (CIN I/II or III) (P=0.001; OR: 3.369; CI:1.634-6.945). Binomial Logistic regression showed that HPV DNA in the blood (P=0.000; OR: 9.324; CI:3.612-24.072) and cervical lesions (P=0.011; OR: 3.622; CI:1.338-9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in blood and cervical lesions (P=0.385). Our results showed that, although there is an association between HPV DNA in the cervix with HPV DNA in blood, only HPV DNA found in the cervix was associated with cervical lesions. |
| Databáze: | OpenAIRE |
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