| Autor: |
T.L. Pazdernik, T. Nishimura |
| Rok vydání: |
1978 |
| Předmět: |
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| Zdroj: |
Journal of Immunological Methods. 23:363-374 |
| ISSN: |
0022-1759 |
| DOI: |
10.1016/0022-1759(78)90211-9 |
| Popis: |
A liquid culture system was developed which supports the production of anti-TNP plaque-forming cells from early precursor cells in the bone marrow of mice. In mouse bone marrow there exists a subpopulation of cells which can be triggered by a polyclonal activator (dextran sulfate) to differentiate into antigen reactive cells (ARC), which can then undergo oligoclonal expansion and maturation in the presence of a thymus independent antigen (TNP-LPS) to yield anti-TNP plaque-forming cells. Under optimal conditions 100–600 anti-TNP plaque-forming cells are obtained per 5 × 10 5 bone marrow cells plated. We refer to these as P-PFC, plaque-forming cells which arise from early precursor cells (pre-ARC) in the bone marrow. The use of hydroxyurea either in vivo or in vitro to destroy cycling cells and Ficoll-metrizoate continuous gradient density columns to obtain bone marrow small lymphocytes suggested that the progeny of cells detected in the P-PFC assay arose from resting small lymphocyte precursors. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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