Development of the MOSFET Type Enzyme Biosensor Using GOx and ChOx

Autor:	Toshifumi Kadokawa, Yoshiaki Hirano, Katsutoshi Ooe, Yasutaro Hamamoto
Rok vydání:	2008
Předmět:	chemistry.chemical_classification Enzyme Materials science General Computer Science Cholesterol oxidase chemistry biology Biochemistry biology.protein Glucose oxidase Electrical and Electronic Engineering Biosensor
Zdroj:	Journal of Robotics and Mechatronics. 20:38-46
ISSN:	1883-8049 0915-3942
DOI:	10.20965/jrm.2008.p0038
Popis:	As the population ages, the management of health is one of the important problems. Before now, we developed the glucose sensor for using at Health Monitoring System (HMS). The whole blood contains the manifold health index markers, and it is very important to measure them. Glucose sensor specifically detects the glucose of the blood, and it monitors the glucose concentration. Our glucose sensor had ‘separated Au electrode’ which immobilized glucose oxidase (GOx). By utilizing this style, it becomes possible that the sensor part is easily miniaturized. In our previous work, GOx was immobilized onto electrode by using of SAMs (Self-Assembled Monolayor) method, and the sensor using this working electrode detected the glucose concentration of glucose aqueous solution. In this paper, glucose sensor which immobilized GOx using the cross-link method was produced. And we carried out operation confirmation of produced glucose sensor using diluted human serum and whole blood. In addition, the cholesterol sensor which immobilized cholesterol oxidase (ChOx) onto separated Au electrode by cross-link method was produced. The immobilization of the ChOx was evaluated from the spectra of ESCA (Electron Spectroscopy for Chemical Analysis), and the performance as a sensor was evaluated.
Databáze:	OpenAIRE
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