Direct in vitro and in vivo monitoring of destruxins metabolism in insects using internal surface reversed-phase high-performance liquid chromatography

Autor:	A. Vey, J.-C. Cherton, P. Cassier, Catherine Lange, M. Pais, C. Mulheim
Rok vydání:	1991
Předmět:	Malpighian tubule system animal structures Chromatography Chemistry Toxin General Chemistry Metabolism medicine.disease_cause High-performance liquid chromatography In vitro In vivo Phase (matter) Hemolymph polycyclic compounds medicine
Zdroj:	Journal of Chromatography B: Biomedical Sciences and Applications. 566:511-524
ISSN:	0378-4347
DOI:	10.1016/0378-4347(91)80268-h
Popis:	High-performance liquid chromatography with internal surface reversed-phase packing provides an analytical tool for studying the in vitro and in vivo metabolism of A and E destruxins in the haemolymph and various organs of male adults of Locusta migratoria. A slight amount of injected E destruxin is shown to be hydrated into E-diol destruxin in the haemolymph. The rest of the toxin is recovered unchanged in the fat-body, pericardial tissues and Malpighian tubules, and some further E-diol destruxin formation occurs in these organs. Because E-diol destruxin is only weakly toxic, this appears to be a detoxication process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2efe57ed7e1998ea4e26ec1b6d1b6793 https://doi.org/10.1016/0378-4347(91)80268-h Zobrazit plný text záznamu