Partial Rescue of the KIT-Deficient Testicular Phenotype in KitW‑v/KitW‑v Tg(TSPY) Mice1
| Autor: | Jörg Schmidtke, Grazia Mauceri, Stephanie Schubert, Britta Marohn, Anja Schöner, Ibrahim M. Adham, Bernhard Vaske |
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| Rok vydání: | 2010 |
| Předmět: |
Genetically modified mouse
0303 health sciences medicine.medical_specialty Transgene Laboratory mouse Stem cell factor Cell Biology General Medicine Biology medicine.disease_cause Phenotype Cell biology 03 medical and health sciences 0302 clinical medicine Endocrinology medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis Internal medicine medicine Carcinogenesis Spermatogenesis Germ cell 030304 developmental biology |
| Zdroj: | Biology of Reproduction. 83:20-26 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.109.082156 |
| Popis: | TSPY encodes the testis-specific protein Y-linked. In man, expression of TSPY is restricted to the testis, where TSPY is expressed in spermatogonia, primary spermatocytes, and round spermatids, and to the prostate gland. There is circumstantial evidence that TSPY is involved in spermatogonial proliferation and gonadal tumorigenesis. Because the laboratory mouse carries the Tspy gene in a naturally silenced state (Tspy-ps), we previously restored TSPY activity in mice and generated a TSPY transgenic mouse line in which the organization and expression of the human TSPY transgene follow the human pattern. In the present study, we generated TSPY transgenic KIT-deficient KitW‑v/KitW‑v mice and analyzed the histology of the testes and epididymides in order to contribute to understanding TSPY function in early germ cell development and spermatogenesis. The KIT receptor and its ligand KITL, previously called stem cell factor, have an indispensable role in hematopoiesis, melanogenesis, and gametogenesis. Homozygou... |
| Databáze: | OpenAIRE |
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